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ATCCCRL-2693 IgG-3B2
基本信息
资源编号 ATCCCRL-2693
资源名称 IgG-3B2
种属 Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
类型 hybridoma: B lymphocyte
形态 lymphoblast
提供形式 frozen
安全等级 1
应用领域 Animals were immunized with a bacterially produced fusion protein expressing pGST-dSREBP (amino acids 1 to 451).
It can be used in immunoblotting assays to detect Drosophila SREBP and to study SREBP in S2 cells grown in various conditions.
培养方法
培养基 The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
传代方法 Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
生长条件 Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
生长特性 suspension
存储条件 liquid nitrogen vapor phase;Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
详细说明
描述 Animals were immunized with a bacterially produced fusion protein expressing pGST-dSREBP (amino acids 1 to 451).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody recognizes both the precursor and mature forms of the Drosophila SREBP transcription factor in cultured S2 (Schneider) Drosophila cells.
It can be used in immunoblotting assays to detect Drosophila SREBP and to study SREBP in S2 cells grown in various conditions.
Derivation Animals were immunized with a bacterially produced fusion protein expressing pGST-dSREBP (amino acids 1 to 451).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed immunoglobulin; monoclonal antibody; against Drosophila sterol regulatory element binding protein (SREBP)
Cellular Products immunoglobulin; monoclonal antibody; against Drosophila sterol regulatory element binding protein (SREBP)
Isotype mouse IgG1 kappa